Regulation of plasmid replication.

INTRODUCTION .............................................. 2 ColEl .............................................. 2 Host Functions Required .............................................. 2 Origin and Direction of Replication .............................................. 3 Primer............................................... 3 Negative Regulation by RNA I .............................................. 3 A Second Negative Regulator .............................................. 4 Summary .............................................. 5 PLASMIDS OF THE FII INCOMPATIBILITY GROUP ........................................... 5 Host Functions Required .............................................. 5 Origin .............................................. 5 Plasmid-Coded Functions............................................... 5 Promoters in the Replication Region ............................................... 6 CopB = RepA2 ............................................... 6 CopA(RNA I=RNA E)............................................... 7 Action of copA ............................................... 7 Product of the copA gene............................................... 7 Target and interaction of CopA ............................................... 7 In Vitro Replication ............................................... 8 Summary ............................................... 8 pT181 ............................................... 8 Requirement for Plasmid-Coded Function ............................................... 9 In Vitro Replication ....................................................... 9 Origin and Direction of Replication ............................................... 9 RepC Protein ........................................................ 9 Negative Regulation of RepC...................................... 9 PLASMIDS OF THE Fl INCOMPATIBILITY GROUP .......................................... 9 Origin and Direction of Replication ............................................... 10 Required Host Factors ............................................... 10 Required Plasmid Functions ............................................... 10 Regulation Is Negative ............................................... 10 Products of the Minimal Replicon ............................................... 10 Sequence Analysis of the Origin Region ............................................... 11 Summary ................................................... 11 REPLICATION OF P1 ................................................... 11 Host Functions Required ............................................... 12 Isolation of Smaller Replicons............................................... 12 Evidence for Negative Regulation of Replication ............................................... 12 Plasmid-Encoded Function(s) Required for Replication .......................................... 12 Repeated Sequences ............................................... 13 Models for P1 Replication Regulation ............................................... 13 R6K............................................... 13 Host Functions Required ............................................... 13 Origin and Direction of Replication ............................................... 13 Replication Terminus and Direction of Replication .............................................. 14 Required Plasmid Function ............................................... 14 Regulation of Pi Protein Production............................................... 14 In Vitro Replication System and Identification of Pi Protein ...................................... 15 Origin Usage In Vitro............................................... 15 Terminus In Vitro............................................... 15 Negative Regulation of Replication............................................... 15 Summary ............................................... 15 COMPARISONS AND CONCLUSIONS............................................... 16 Importance of RNA Secondary Structure ............................................... 16 Efficient Utilization of DNA............................................... 16 Alternative Origins ..................................... 16 Secondary Limitations on Plasmid Copy Number .................................... 17